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Ligation might not be adequate due to the thick gastric mucosa

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The overall pathway for EPA and DHA synthesis involves three endoplasmic reticulum (ER) fatty acid elongation enzyme systems, three desaturation reactions involving two different desaturases, and a peroxisomal -oxidation step as the final step in DHA synthesis as outlined in the Figure below

Dil-LDL uptake assay Huh-7 cells were seeded in black transparent-bottom 96-well plates for 24 h and treated with experimental reagents for an additional 24 h