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The His6-SUMO tag was cleaved by ULP1 treatment, and the tag-free protein was fractioned with Ni-NTA column and Superdex 75size exclusion column (GE Healthcare) with 25 mM HEPES (pH 8.0), 300 mM NaCl, and 1% glycerol

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To examine the accuracy of transcript-level expression profile, the gene-level profile of 6 DEGs which linking pco105094 and umc2770 with qRT-PCR were compared to those of DEGs with RNA-seq

10.1038/s41467-019-08936-1 [DOI] [PMC free article] [PubMed] [Google Scholar] 226.Chen J, Zhang S, Wu J, Wu S, Xu G, Wei D

Stable gastric pentadecapeptide BPC 157: novel therapy in gastrointestinal and other tissue healing models