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(2023) 120:e2211937120

E., & Labarre, J

The qPCR reaction mixture was prepared as follows: 7.5 L of 2 Universal Blue SYBR Green qPCR Master Mix, 1.5 L of 2.5 M gene primers (forward + reverse), 2.0 L of cDNA, and 4.0 L of nuclease-free water

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These include some cases of Diamond-Blackfan anemia , in which mutations in a ribosomal RNA processing gene (RPS19) result in red cell hypoplasia