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Add solvent slowly along the inner wall of the vial to minimize foaming or peptide denaturation

MOTS-c (mitochondrial open reading frame of the 12S rRNA-c) is a short peptide naturally produced by mitochondria

Clean the chosen site with an alcohol swab in a circular motion, starting from the center and spiraling outward

The gut microbiota-derived deamidated pathway facilitates the utilization of NAM or NR for hepatic NAD + synthesis, suggesting that manipulation of microbiota might offer a new option to manipulate NAD + metabolism

Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully [13]