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IF and IHC staining of tissue sections Tumour tissue sections were deparaffinized in Histo-Clear (Scientific Laboratory Supplies) for 5 min, rehydrated through a series of decreasing ethanol concentrations, followed by a final 5 min wash in distilled water (dH 2 O)
unc-119(+)]) worms, which bear a dopaminergic neuron targeted CL-1 degron ubiquitination signal translationally fused to CFP, were used for a minimum of three replicate series per treatment group (chronic metabolite and/or pharmacological treatment) at day 7 post hatching
The cause of E2 deficiency includes point mutations and mRNA mis-splice events
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