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Qualitative assessment of nitric oxide production by fluorescent dye 3-Amino-4-aminomethyl-2,7-difluorescein, diacetate (DAF-FM DA, Sigma) HUVECs grown in M199 based culture medium was stimulated with 1.0 g/ml BPC 157 for 30 min to induce the nitric oxide generation

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It has FDA approval, a well-characterized safety profile, and proven efficacy for its specific target

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For musculoskeletal injuries, that means the patient still needs proper diagnosis, load management, progressive rehabilitation, adequate protein intake, high-quality sleep, and correction of the movement pattern or training error that contributed to the injury