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Assay reagents and procedures IMAP Assay The 5 L of 4X compound solution, 5 L of 4X substrate/ATP/Metal solution, and 10 L of 2X kinase solution were prepared with assay buffer (20mM HEPES, 0.01% Tween-20, 2mM DTT, pH7.4) and mixed and incubated in a well of polystyrene 384 well black microplate for 1 hour at room temperature

Andrea Cordes, Mrs

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This indicates that the compound did not kill the cells (Figure 3, Panel A, and Figure 4, Panel A)

Recently, the groups of Lu and Liu developed an integrated computational and experimental approach coupled with yeast display, that enable massive parallel screening of protein sequences and structures 88