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The irony was not lost on clinicians or patients: the FDA restricted access through the most regulated channel while the least regulated channel remained wide open

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NEM was added to cell lysates to a final concentration of 50 mM immediately after collection to prevent post-lysis glutathionylation by blocking free thiols in both proteins and BioGEE and also prevent thiol-disulfide exchange reactions that could result in loss of glutathionylation

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