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Whole-heart ex vivo left anterior descending coronary artery ligation on a Langendorff system To measure the infarct size in a whole-heart ex vivo model, the left anterior descending (LAD) coronary artery was ligated for 40 min, following 1 h of equilibration on a Langendorff perfusion system, perfused at 8 mL/min at a temperature of 37C and bubbled with 100% O 2

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