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Description
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A full-length ccz-1 cDNA was amplified from pDONR/Zeo- ccz-1 using P1119 AACGGCGACTGGCTGG CC ATGGAGTCGATTGCAAATCCATTG 3 and P1120 5 CTTGGCTGCAGGTCGAC GG TCAACTAAAAAATATGGCTTCGAAATGGG 3 with Q5 High Fidelity DNA polymerase (NEB) and inserted into pEG202 digested with EcoRI and XhoI, using NEBuilder HiFi DNA Assembly Cloning as described by the manufacturer (NEB)

B., Martin, D

Because nutrient relationships can be complex, its best to follow veterinary guidance rather than building a stack on your own

Mutation research Reviews in mutation research
