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Hi all, I am a 21 y/o male who eats meat and was diagnosed with a B12 deficiency (not PA) about 2 months ago

A set of no-cell control group containing only the culture medium without cells were prepared to serve as negative control to determine background fluorescence that might be present

Currently, SA--gal activity is considered one of the most crucial markers of cellular senescence [26]

The reaction buffer consisted of RB + BSA with 1 U/ml hexokinase, 2.5 mM d-glucose, 2.5 U/ml G-6-PDH, 2.5 mM NADP + , 10 mM succinate, 10 mM glutamate, 0.5 mM malate, and 10 M cytochrome c (all reagents from Sigma)