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BSA typically maintains a high -helical content under physiological pH, i.e., close to neutral pH, but acidic or basic environments can partially unfold the protein, decreasing the -helix content

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Animal experiments have used iPS cells as a cell source and induced differentiation into notochordal cells (Sheyn et al., 2019), nucleus pulposus-like cells (Zhang et al., 2020), or cartilaginous tissue ( 5 Biomaterials and scaffolds Biomaterials aimed at NP replacement in IVD regeneration, comprising synthetic and biological materials designed to restore disc height and segmental stability without compromising the annulus fibrosus, are predominantly injectable materials (Mehrkens et al., 2012)

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