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The most consistent finding: researchers who dose MOTS-c in the morning during fasted states report more pronounced shifts in substrate utilizationmeasured via respiratory exchange ratio (RER)than those administering it post-meal

Data were low-pass filtered at 2 kHz and a 5-ms template (2 ms baseline, 2 pA amplitude, 0.5 ms rise time, and 1 ms decay time) was used to detect spontaneous EPSCs