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All cell lines, except Jurkat and THP-1, were maintained in DMEM high glucose (4.5 g l 1 glucose, 21969-035, Gibco) supplemented with 10% fetal bovine serum (FBS), 2 mM l-glutamine, and 1% penicillin/streptomycin at 37 C with 5% CO 2 , unless stated otherwise

Detoxification of patients including withdrawal of the overused drugs and treatment of withdrawal symptoms (which often includes a transient worsening of headache) may be necessary

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