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Quantitative real-time PCR mRNA was extracted using the Cells-to-Ct kit (Ambion)

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PMID: 15703015

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The high concentrations of glutathione in GCF in health and extracellularly in the lung are similar, and therefore may represent an important AO and anti-inflammatory (anti-proinflammatory cytokines) defense strategy common to exposed epithelial cells.[29] Both sites consist of fluid containing high concentrations of glutathione, and in chronic inflammatory conditions, glutathione concentrations are reduced in both sites, owing to the failure of normal tissue homeostatic mechanisms to protect against the host-mediated tissue damage