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Western Blot analysis Livers from F0-CON and F0-HFD mice were homogenised in RIPA buffer

Time-lapse microscopy For videos of Sema4D-Fc-647 endocytosis, HeLa cells expressing GFP-tubulin and mCherry-Histone plated in chamber slides were incubated with Sema4D-Fc-Alexa647 in FluoroBrite DMEM Media (Thermo Fisher Scientific) with 10% serum, 1 mM sodium pyruvate and 2 mM glutamine and imaged using a Nikon Inverted spinning disk confocal microscope at 40

Wang X, Megert B, Hellwig E, Neuhaus KW, Lussi A

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