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Protein concentration was quantified with a BCA protein assay kit (Thermo Fisher, 23227) and lysates were then mixed 1:1 with 2x Laemmeli buffer and boiled for 5 min at 95 C

The authors attempted also to elucidate the underlying mechanisms highlighting that H19 directly interacted with the Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (4E-BP1), at the TOS motif inhibiting competitively its binding to Raptor

Of note, oxalate is thought to be responsible for the cold-induced hypersensitivity observed in oxaliplatin-treated, but not cisplatin-treated, patients [10, 11], although additional mechanisms, including the binding of platinum complexes to cellular proteins, have also been suggested [12]

In animal model (aged mice), apelin stimulates autophagy in myofibers, reduces inflammation, and supports muscle repair

Peptide therapy continues as the focus of a great deal of research effort particularly as it shows promise in the care of heart conditions