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Several amino acids had their concentrations increased following MTX treatment: glycine, alanine, phenylalanine, threonine, histidine, valine, arginine, leucine, isoleucine, tyrosine, and proline

doi: 10.1038/ncomms11100, 63 Monte-SilvaK.KuoM

et al., 2023)

Instead, they are usually given in concentrated doses of 1,000 mcg (1 mg)
