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It can be fixed with aldehydes and permeabilized by Triton X-100 (0.5%)

Membranes were closed in Tris-buffered saline (TBS, pH 7.4) containing 5% skim milk and 0.1% Tween 20, washed twice in TBS containing 0.1% Tween 20, and incubated overnight at 4 C with primary antibody, followed by the secondary antibody for 1 h at room temperature