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Eukaryotic Resistance Genes (e.g., Puromycin/Blasticidin): Enable effective antibiotic selection, eliminating non-transduced cells

During these life stages, hyperinsulinemia is considered as part of the physiological responses against reduced insulin sensitivity elicited by growth hormones 85,86,87

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Therefore, it would not be wrong to assume that the resulting phenotype can be attributed to the combined effect of altering the specific coding gene as well as the other non-coding genes that get affected inadvertently due to the disruption by genetic engineering method used to generate the knockout organism