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Description
To determine the intracellular glutamate concentrations, a Glutamine/Glutamate-Glo Assay (Promega) was used following the manufacturers instructions
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However, some peptides are poorly soluble at neutral pH and require a mildly acidic solvent such as 0.6% acetic acid water
After this period, you must dispose of the vial responsibly to prevent any risk of bacterial contamination
