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The samples were subjected to click reaction by adding 1 l rhodamine azide (10 mM in DMSO), 5 l BTTAA (10 mM in DMSO), 2 l CuSO 4 (50 mM in ddH 2 O) and 2 l NaAsc (100 mM in ddH 2 O) to 90 l of each sample and incubated for 1 h at r.t

Although not evaluated here, lower intracellular GSH levels would also deactivate the GSH-dependent GPX4, further enhancing ferroptotic cell death (8, 38)

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Although the current evidence is very promising, there are still some knowledge gaps that should be addressed to further clarify the exact mechanisms linking NNMT to obesity and T2D and to develop clinical drugs targeting NNMT

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