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For ELISA, liver tissues were sonicated in 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100 and protease inhibitor cocktail (EDTA-free Complete Mini, Roche)

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Moreover, magnolol raises the levels of BDNF expression in the serotonergic system in the brains of rats who have experienced unpredictable chronic mild stress [196]

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Relative number of viable cells was normalized to the DMSO-treated control cells