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Water is essential for producing saliva and gastric juices, which break down food, as well as lubricating the digestive tract

One of the most frequently employed animal stress models is the restraint stress model leading to severe stress gastric lesions in rats

B., Follett, K

After denaturing in a boiling water bath, the proteins were separated by SDSpolyacrylamide gel electrophoresis (SDSPAGE), transferred to a PVDF membrane, blocked with 5% milk in TBST for 30 min at room temperature, and then incubated with primary antibody at 4 C overnight with shaking

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