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CLSM images were obtained using an exciting laser at the wavelength 488 nm and the fluorescence detection band was set to 500650 nm for rhodamine green using photomultiplier tubes (PMT), while the bright-field images were recorded simultaneously with the CLSM images using transmission PMT

S3a,b) but the GSH basal transport, as expected, was lost (Supplementary Fig

The stained tissues were mounted in Vectorshield (Vector Laboratories) and were observed under a microscope (Carl Zeiss AG, Oberkochen, Germany)

An important caveat upfront: most research covers IGF-1 broadly, not the LR3 analog specifically

These methods demonstrate exceptional sensitivity and have been successfully applied to the analysis of human plasma samples and liver homogenates, making them particularly valuable for clinical research applications