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The following primers were used to select positive embryonic stem cell clones H052.17: binds to the elongated short arm of homology 5-CACGCTGAACTAGCATAGCC-3 H052.19: binds to the Cre-ERT2 5-CTACACCAGAGACGGAAATCCATC-3

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BourneJHSuthyaARWanrooyBJWilsonJLWenSWBastowCRet al

Transduction efficiency was measured by FACS analysis, which demonstrated an efficiency of ~30% for all inserts, representative of one integration site per cell

Proliferation of human hematopoietic progenitors in long-term bone marrow cultures in gas-permeable plastic bags is enhanced by colony-stimulating factors