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Preclinical research commonly uses 250500 mcg per day for musculoskeletal applications [3] [4]
Therefore, DA would presumably inhibit SS release at least at two levels (the hypothalamus proper and the median eminence)
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Plasma samples were diluted with three volumes of ethanol containing 1% formic acid, and the resultant supernatant was subjected to online sample clean-up by turbulent flow chromatography on a Cyclone (50 0.5 mm) column from Thermo Fisher prior to LCMS analysis
