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Published cosmetic studies on GHK-Cu skin effects used daily application over 8-12 week periods before assessing results

Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders (BenchMark, Invitrogen, and Kaleidoscope, BioRad)

[DOI] [PMC free article] [PubMed] [Google Scholar] 80.Andersen G., Meiffren G., Famulla S., Heise T., Ranson A., Seroussi C., Eloy R., Gaudier M., Charvet R., Chan Y., et al

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Our primary outcome of interest was the GSQS total score