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The primers were optimized for suitable annealing temperature, amplification conditions, incubation time (for RPA), the specificity and the sensitivity were calculated

[DOI] [PubMed] [Google Scholar] 13.Ryder JW, Portocarrero CP, Song XM, Cui L, Yu M, Combatsiaris T, et al

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Melasma is an acquired chronic dyschromia, more prevalent in menopausal women and characterized by cutaneous hypermelanosis in sun-exposed areas, especially the face

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