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2 mM 2-mercaptoethanol, 0.01% NP-40) for two times, uS10 was eluted from beads by incubation with 600 L of Elution Buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl, 10 mM MgCl 2 , 300 mM Imidazole

Accessed 11/4/2025, Menezo Y, Elder K, Clement A, Clement P

This enzyme mediates the inflammatory response by producing pro-inflammatory cytokines, such as IL-1 and IL-18, which contribute to the development of acne vulgaris (180)

Brock, K.P
