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Equal amounts of protein (1020 g) in 1 Laemmlis buffer were separated on sodium dodecyl sulfate polyacrylamide gel electrophoresis using 7.512% gels and transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad)
After that, LLO was added to a final concentration of 5 M and the sample mixtures were further incubated for 60 minutes
Melnyk S, Fuchs GJ, Schulz E, Lopez M, Kahler SG, Fussell JJ, et al
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