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the FmocFmoc20PIPTFA In addition, in the process of the present invention, a mild Fmoc route is adopted, and only 20% of PIP is used for each step of removing Fmoc, and the condensation reaction time of each step is short, which greatly shortens the production cycle

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Then, 10 g of peptides from the HEK-293T cell digests, with or without SCX pre-fractionation, were loaded in the loading solvent (95% MeCN, 0.1% TFA) to the homemade SPE columns

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