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In the 12th week post-surgery, the rats were euthanized under deep anesthesia with pentobarbital sodium, and the right knee joints and blood were obtained for subsequent experiments

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In addition, Mn overexposure may affect tyrosine hydroxylase (TH), an enzyme required for dopamine synthesis in dopaminergic terminals

Synthesis and Release of Thyroid Hormones Hormones are produced in the colloid when atoms of the mineral iodine attach to a glycoprotein, called thyroglobulin, that is secreted into the colloid by the follicle cells