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The ETC is the principal source of mitochondrial ROS

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Ubiquitin constructs used include WT (wild-type), K48R, K63R, K48 (all ubiquitin lysines mutated to arginine except K48) and K63 (all ubiquitin lysines mutated to arginine except K63)

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[1] Fractional carbon dioxide lasers and Nd:YAG laser therapies are also effective