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Smart and responsive systems: pH-sensitive hydrogels and enzyme-responsive nanocapsules release drugs selectively in acidic or inflamed microenvironments, enhancing precision (Wang et al., 2025

After being co-incubated with various formulations for predetermined time at 37 C, the cell culture medium was washed away with PBS

Next, cells were washed with PBS, incubated for a further 6 to 12 h, lysed, and bacterial enumeration was conducted to assess the internalization capabilities

Charecterising the specificity of the biosensor To confirm that glycine was activating the biosensor and not any other amino acid, the transformed bacteria was exposed to varying concentrations of an amino acid that should not initiate a response