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To construct a pTRV2- TDR4 recombinant, a 360-bp Eco RI/ Bam HI-containing DNA fragment of the TDR4 gene, corresponding to nucleotides 323682 (NM_001247244.2), was amplified from tomato fruit complementary DNA (cDNA) using primers TDR4 -VIGS-For and TDR4 -VIGS-Rev (Supplementary Table S1)

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