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Description

Cell 60 GaschlerM

The exclusion criteria were subjects with distant metastatic cancer, subjects undergoing immunotherapy, oral Tyrosine kinase inhibitors (TKI), or chemotherapy, participants who are being treated with additional experimental agents, uncontrolled concurrent illness that would make it more difficult to follow research guidelines, history of New York Heart Association Class III or IV heart failure, a myocardial infarction within the last six months, an uncontrolled cardiac arrhythmia, use of Luteinizing hormone-releasing hormone (LHRH) agonists, antagonists, Gonadotropin hormone-releasing hormone (GNRH) analogs, and antiandrogens in androgen deprivation therapy (ADT) or antiandrogen therapy and those subjects using Coumadin or Warfarin

MS measurement Proteins were extracted from the retinal tissue of these individuals using the detergent sodium dodecyl sulfate (SDS), which is the most efficient reagent for lysing tissue and cells to achieve complete protein extraction [37]