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The signal transduction of TGF- can stimulate diverse cellular responses and is particularly critical to embryonic development, wound healing, tissue homeostasis, and immune homeostasis in health

Add the standard solutions to each well of the microplate to generate the standard curve
The broodstock were injected with 10 g/kg of a Luteinizing hormone-releasing hormone (LHRH) analog after the 84-day feeding period to induce spawning

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