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Description
Having incubated the immunoprecipitated samples overnight at 4 C with rotation, the anti-M2 FLAG antibody-conjugated agarose beads were washed three times (each for 5 min) with ice-cold washing buffer (50 mM Tris, pH 7.4, 137 mM NaCl, 10% glycerol, 1% NP-40)
Nagle P, OSullivan E, Hathaway BJ, Muller E: Crystal structure of bis (2,2-bipyridyl) monochlorocopper (II) tetrafluoroborate
Not necessary, but refrigeration is acceptable if it prevents temperature fluctuations (never freeze)
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Tozzi, M
