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In contrast, application of EEM fingerprints for the development of UPLS-DA model allowed for ideal identification of serotonin (precision, sensitivity, and specificity were 1.000 for both train and test set

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(PubMed) Wald DS, Law M, Morris JK

Thereafter, trypsin at 1:50 was poured for overnight digestion and that at 1:100 was introduced for additional 4 h of digestion, followed by peptide desalting using a C18 SPE column

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