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Activated microglia and astrocytes release proinflammatory cytokines including tumor necrosis factor-alpha (TNF- ), interleukin-6 (IL-6), and interleukin-1 (IL-1), creating a hostile neuroimmune milieu ( In PD brains, persistent glial activation is associated with regions of dopaminergic degeneration, indicating a spatial correlation ( Epigenetic regulation also influences proteostasis in PD

A variant of these induction protocols is the so-called pairing protocol, whereby during low-frequency activation of axons (0.11 Hz), individual cells are held at depolarized membrane potentials by passing current through the recording electrode