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Signal 3 was initially conceptualized as the direct sensing of inflammation through cytokines necessary for full T cell activation and differentiation

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doi: 10.1186/s40104-017-0163-7 8 PathakRPrasadSKumaresanAKaurMManimaranADangAK

Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein

The hypoxia/reoxygenation (H/R) in vitro model was established by placing the cells in a hypoxia condition (4% N 2 /5% CO 2 /1% O 2 ) for 12 h and then subjecting them to reoxygenation (5% CO2/95% air) for 12 h, prior to pretreatment with 6-gingerol, MitoTEMPO, or transfection