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The cell pellet was suspended in 10% ice-cold TCA and centrifuged for 15 min at 9,000 g

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doi:10.1161/CIRCRESAHA.109.198366, PMID: 19910579 Starkov AA, Fiskum G, Chinopoulos C, Lorenzo BJ, Browne SE, Patel MS, Beal MF (2004) Mitochondrial alpha-ketoglutarate dehydrogenase complex generates reactive oxygen species

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As I elaborate on in my video The Symptoms of Vitamin B12 Deficiency , these include: Abdominal distention Chronic diarrhea Shortness of breath Swollen, red, painful feet Skin darkening Bilateral useless hand syndrome Numbness and tingling in the hands and feet Muscle cramps Dizziness Cognitive disturbances Difficulty walking Erectile dysfunction Fatigue Depression Psychosis Coma Blindness Vitamin B12 Deficiency Treatment Is A Vitamin B12 Shot Better Than Taking B12 Orally