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This priming-dependent enhanced phosphorylation aligns with our in vivo post-translational modification analysis: S114 phosphorylation is consistently detectable, whereas downstream residues S117 and S120 remain elusive, likely due to the transient nature of their phosphorylation following S114 priming

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The tissue was rapidly frozen in OCT gel and sectioned into 10-m thickness

L-carnitine C
