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Growth factor-derived HLCs were maintained in medium consisting of Williams E Medium, 10% FBS, 1% GlutaMax, 1% NEAA, 1% Penicillin/Streptomycin (Thermo Fisher Scientific), 1% Insulin-Transferrin-Selenium supplement (Sigma), 10 7 M dexamethasone (Sigma Aldrich)

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LF consists of a simple polypeptide chain that consists of two globular lobes with a carboxyl (C) and an amino (N) end connected by an helix

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Research demonstrates that Dihexa binds to hepatocyte growth factor (HGF) with picomolar affinity and activates the c-Met receptor system, promoting formation of new synaptic connections in the hippocampus