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For one-step reactions, all components were mixed in 96-well microtitre plates in 100 l volume (PS, F-Bottom, clear, Greiner) and reactions were performed for 30 min in a Spark 20 M (Tecan) plate reader at 30 C
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This minimally invasive procedure helps restore confidence by reducing visible veins and improving overall skin clarity

Both approaches are producing results that seemed impossible just a decade ago