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Description
Mitochondrial COA7 is a heme-binding protein with disulfide reductase activity, which acts in the early stages of complex IV assembly

281 Even when implemented without automation, the process of producing 0.11 mg of purified protein via CFE, from generating DNA template to purifying the protein of interest, can require similar effort to completing a plasmid purification kit

[PubMed: 22832530] 166

Spectrophotometric assays Spectrophotometric assays are known for their user-friendly nature, requiring minimal amounts of reagents while also being applicable in high-throughput screening (Prodanovi et al

doi: 10.1016/j.phytochem.2010.01.005 108 LiuX.QuanW.BartelsD
