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To measure ROS levels, cells were incubated with 5uM cellROX Deep Red (ThermoFisher, #C10422) for 30 min in serum-free medium at 37 C for 30 min in normoxia or hypoxia
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collected data and contributed to writing the manuscript
Thirdly, activated mTOR enhances wound healing in DFU by upregulating the expression of multiple growth factors, 83 regulating the accumulation of lipids in endothelial cells and fibroblasts, 84 inducing epithelial-mesenchymal transition, 85 and promoting the proliferation and differentiation of fibroblasts
GSE227819 [71]
