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At the cellular level, Mg deficiency triggers secondary messengers, such as ROS, which activate stress signaling networks, whereas Mg sufficiency reduces ROS accumulation by upregulating antioxidant enzymes, such as SOD and CAT ( 6 Magnesium and plant abiotic stress tolerance In the context of abiotic stress, Mg plays a vital and multifaceted role in enhancing plant tolerance

Science 329 , 946949 (2010)

To minimize sample variations, the mRNA expression of the target gene was normalized relative to the expression of the actin housekeeping gene

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Solasonine, a steroidal alkaloid, is derived from Solanum nigrum L