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Factors that accelerate expiration Temperature fluctuations: Repeated freeze-thaw cycles very damaging Each thaw = period of degradation One freeze-thaw: Minor impact Multiple cycles: Significant loss of potency Keep frozen continuously until ready to reconstitute Light exposure: UV and visible light degrade peptides Some peptides more photosensitive (GHK-Cu) Amber vials protect partially Keep vials in boxes or dark storage Air exposure: Oxygen causes oxidation Minimize time vial is open Don't store without cap Use quickly after reconstitution Moisture exposure (for powder): Even atmospheric humidity can start degradation Keep in sealed vials always Don't open unnecessarily Store in dry environment Contamination: Non-sterile technique introduces bacteria Bacterial enzymes destroy peptides rapidly Always use alcohol wipes Never touch rubber stopper Use bacteriostatic water to inhibit growth pH extremes: Some peptides unstable at wrong pH Reconstitute with appropriate solution Bacteriostatic water usually optimal (slightly acidic) Don't mix with other substances without knowledge Shelf life before reconstitution (lyophilized powder) How long dry peptides last in different conditions

The Endocrine System 17.3 The Pituitary Gland and Hypothalamus Learning Objectives By the end of this section, you will be able to: Explain the anatomical and functional relationships of the hypothalamus and the posterior and anterior lobes of the pituitary gland Identify the two hormones released from the posterior pituitary, their target cells, and principal actions Identify the six hormones produced by the anterior lobe of the pituitary gland, their target cells, their principal actions, and regulation by the hypothalamus Summarize the direct and indirect effects of growth hormone Explain how growth hormone release is regulated by hypothalamic hormones The hypothalamuspituitary complex can be thought of as the command center of the endocrine system

Taking Glutathione at Night While the morning is about protection, the night is about restoration